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Thursday, December 5, 2013

Testing for Food Purity

The point of this lab was to study a technique of immunology and apply it in a test for food purity. Immunology is the study of an organism's response to a foreign organic substance or antigen.

We acquired an agar plate divided into three equal zones. And we split the plate between two other groups.

In our division of the plate we had to make four different wells in the agar. To do this we used a dropping pipet. And we first squeezed it letting out all the air, then inserted it into the agar and released the bulb to we then sucked up the small circle piece we just separated. We are arranged the four wells according to this diagram.

Once we finished this we first filled well number one with Hamburger Extract. Following the instructions we proceeded to put goat anti-horse albumin in well 2, goat anti-bovine albumin in well 3,and goat anti-swine albumin in well 4.
Once the other people put their solutions in their section of the agar plate, we placed the plate in a drawer face up to "incubate it". It had to be placed face up so that the solution that we put in the wells wouldn't pour out.

When we returned to lab in Thursday we found that our results we non-conclusive, or they did not work as they we expected to. This could have been from old solutions that we were using or some other factors.





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