Disclaimer
All content provided on this blog is representation of the blog owner and not Franciscan University of Steubenville. The information on this site is purely used for education purpose. The owner of this blog makes no representations as to the accuracy or completeness of any information on this site or found by following any link on this site. The owner will not be liable for any errors or omissions in this information nor for the availability of this information. The owner will not be liable for any losses, injuries, or damages from the display or use of this information.
Privacy
The owner of this blog does not share personal information with third-parties nor does the owner store information is collected about your visit for use other than to analyze content performance through the use of cookies, which you can turn off at anytime by modifying your Internet browser’s settings. The owner is not responsible for the republishing of the content found on this blog on other Web sites or media without permission.
Blog Comments
The owner of this blog reserves the right to edit or delete any comments submitted to this blog without notice due to;
1. Comments deemed to be spam or questionable spam
2. Comments including profanity
3. Comments containing language or concepts that could be deemed offensive
4. Comments that attack a person individually
This policy is subject to change at anytime.

Thursday, December 5, 2013

Antibiotics!!

As nurses we are going to have to deal with the different kinds of antibiotics all the time!
So for this lab experiment we tested different antibiotics and how the worked on our unknown bacteria.
We had already become familiar with the antibiotic Bacitracin  used for Streptococcus, or strep throat infection.

We first prepared a smear plate of our unknown bacteria using the aseptic technique.

Next using forceps, that were both sterilized in the flame of a bunsen burner and by spraying them with 70% ethanol, we removed a disc of each antibiotic and placed it is one of the five sections we divided the nutrient agar plate into.
We used: novobiocin, erythromycin, neomyosin, tetracycline, and penicillin.
We then put it in the incubator at 37 degrees centigrade, and waited to see what the results might be.



When we returned to class the next time we discovered which antibiotics worked the best.

Below are the results of our test:





















As you can see all of the different antibiotics killed our bacteria. The neomyosin, however, did not work as effectively as all the other antibiotics because there was still bacteria growing on the sides of that section. The diameters for the antibacterial agents are as follows: penicillin has a diameter of 45 millimeters, tetracycline has a diameter of 45 millimeters, novobiocin has a diameter of 45 millimeters, erythromycin has a diameter of 45 millimeters, and finally neomyosin has a diameter of 30 millimeters. After determining the diameters, we can determine whether our bacteria was susceptible or resistant to the antimicrobial agents we tested. For penicillin the diameter has to be greater than 29 millimeters, for tetracycline the diameter has to be greater than 19, for novobiocin the diameter has to be greater than 22 millimeters, for erythromycin the diameter has to be greater than 23 millimeters, and finally for neomyosin the diameter has to be greater than 17 millimeters. Since all of our diameters are greater than their respective range this tells us that our bacteria is susceptible to all of the antimicrobial agents that we tested.

Therefore, we could say that if we obtain this disease we could easily kill the bacteria by virtually all the antibiotics that we tested!  
Posted by

No comments:

Post a Comment

Subscribe to: Post Comments (Atom)