Sample S!

Dr. Pathakamuri gave each group a pure sample of an unknown bacteria in an agar slant. We put this unknown culture in an incubator at 37 degrees Celsius. The next day in class we came back to find that our bacteria had grown in a zigzag pattern in the tube.

Our next step was to obtain another tube of this pure culture so we could have both a working sample and a stock sample of our bacteria.

In order to do this we had to hold both the slanted agar with the bacteria already in it and the clean slanted agar in one hand and the sterilized inoculating loop in the other hand.

 Without setting them down we took the caps of both tubes off and held then in the hand with the inoculating loop. We flamed the mouth of the tube, and then put our sterilized loop in the slanted agar with the bacteria and grabbed a bead of culture. We transferred this bacteria to the clean slanted agar and spread it in a zigzag motion from the bottom of the tube up. After this we flamed the openings of the tubes and recapped them and then sterilized the loop.