Culturing Anaerobic Bacteria!

In a previous test we found out that our bacteria was anaerobic, but we were not yet sure if it was facultative or a strict anaerobe. But we were going to find out.
A strict anaerobic bacteria is one that cannot grow in the presence of oxygen.
To determine this we used the GasPak Anaerobic system. Everyone in the class made a smear plate of their bacteria and put it into the container.

We then put some thermo-packs into the container, and an anaerobic indicator strip so be could see when it turned blue that there was no more oxygen left in the container.
Then we sealed the container with a screw cap, closing it tightly so no oxygen could get in.
We then incubated our bacteria at 37 degrees centegrade, and waited to see what our results might be.

When we took the GasPak out of the incubator and obtained out bacteria samples from it, we found out that our bacteria was not a strict anaerobe, because it did not grow in the container.

We instead determined that our bacteria was facultative anaerobic.