For our blood agar plate, we determined that our bacteria do not secrete enzymes that completely dismantle the red blood cells, also known as gamma-hemolysis.
For the Eosin Methylene Blue (EMB) plate, we found that since our bacteria is gram-negative, our bacteria grew a little bit.
For our Mannitol Salt Agar, we found out that our bacteria cannot live in a high salt environment since our bacteria did not grow on the agar plate. This means that our bacteria are not halophile.
For our MacConkey Agar plate, we determined that our bacteria can not ferment lactose because they are not able to grow on this medium.
For the Phenylethyl Alcohol (PEA) Agar, we found that our bacteria grew a little bit on the agar plate, which was a little weird considering we thought our bacteria are gram-negative and PEA inhibits the growth of gram-negative bacteria.
In our thioglycollate broth tube, our bacteria grew in a pretty cool way where it grew right in the middle of where oxygen is not present in the tube. Our bacteria are anaerobic but it is amazing to see our bacteria floating in the middle of our tube!
For our DNA Hydrolysis test, we needed to flood the plate with 1N HCl (hydrochloric acid) to determine if our bacteria are able to hydrolyze (digest) DNA.
After adding 1N HCl since there is no clear area around our bacteria growth we determined that our bacteria are not able to hydrolyze (digest) DNA.
Conclusion:
After we looked over all of the results, we found that some of the results came out weird considering that we thought our bacteria was gram-negative. So our professor told us to do another gram-stain of our bacteria.
After doing another gram-stain of our bacteria, we found that our bacteria has both gram-negative and gram-positive stains (as shown above). We showed the gram-stain to our professor and he determined that our bacteria are more gram-positive than gram-negative BUT! our bacteria are able to take on both stains, which makes it gram-variable also. Therefore, our results were a little weird because our bacteria are gram-positive to gram-variable. Our bacteria seems to not like to make up it's mind!
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