Disclaimer
All content provided on this blog is representation of the blog owner and not Franciscan University of Steubenville. The information on this site is purely used for education purpose. The owner of this blog makes no representations as to the accuracy or completeness of any information on this site or found by following any link on this site. The owner will not be liable for any errors or omissions in this information nor for the availability of this information. The owner will not be liable for any losses, injuries, or damages from the display or use of this information.
Privacy
The owner of this blog does not share personal information with third-parties nor does the owner store information is collected about your visit for use other than to analyze content performance through the use of cookies, which you can turn off at anytime by modifying your Internet browser’s settings. The owner is not responsible for the republishing of the content found on this blog on other Web sites or media without permission.
Blog Comments
The owner of this blog reserves the right to edit or delete any comments submitted to this blog without notice due to;
1. Comments deemed to be spam or questionable spam
2. Comments including profanity
3. Comments containing language or concepts that could be deemed offensive
4. Comments that attack a person individually
This policy is subject to change at anytime.

Monday, November 25, 2013

Hmmm....

The results of all our tests seem a little strange considering that we believed that our bacteria was gram-negative.

For our blood agar plate, we determined that our bacteria do not secrete enzymes that completely dismantle the red blood cells, also known as gamma-hemolysis.


For the Eosin Methylene Blue (EMB) plate, we found that since our bacteria is gram-negative, our bacteria grew a little bit.


For our Mannitol Salt Agar, we found out that our bacteria cannot live in a high salt environment since our bacteria did not grow on the agar plate. This means that our bacteria are not halophile. 


For our MacConkey Agar plate, we determined that our bacteria can not ferment lactose because they are not able to grow on this medium. 


For the Phenylethyl Alcohol (PEA) Agar, we found that our bacteria grew a little bit on the agar plate, which was a little weird considering we thought our bacteria are gram-negative and PEA inhibits the growth of gram-negative bacteria.


In our thioglycollate broth tube, our bacteria grew in a pretty cool way where it grew right in the middle of where oxygen is not present in the tube. Our bacteria are anaerobic but it is amazing to see our bacteria floating in the middle of our tube!


For our DNA Hydrolysis test, we needed to flood the plate with 1N HCl (hydrochloric acid) to determine if our bacteria are able to hydrolyze (digest) DNA.


After adding 1N HCl since there is no clear area around our bacteria growth we determined that our bacteria are not able to hydrolyze (digest) DNA.


Conclusion: 
After we looked over all of the results, we found that some of the results came out weird considering that we thought our bacteria was gram-negative. So our professor told us to do another gram-stain of our bacteria. 

After doing another gram-stain of our bacteria, we found that our bacteria has both gram-negative and gram-positive stains (as shown above). We showed the gram-stain to our professor and he determined that our bacteria are more gram-positive than gram-negative BUT! our bacteria are able to take on both stains, which makes it gram-variable also. Therefore, our results were a little weird because our bacteria are gram-positive to gram-variable. Our bacteria seems to not like to make up it's mind! 

No comments:

Post a Comment