First we obtained a Simmons citrate slant tube and used an aseptic technique to inoculate the agar slant with a loopful of bacteria from our agar slant culture.
We then incubated the inoculated slant at 35 degrees Celsius for about 2 days.
When we reviewed the results, we found out that out test was negative. Meaning that our bacteria does not utilize nitrate.
The color of the agar slant remained green instead of changing a bluish color which would have indicated that our bacteria utilized nitrate.
We repeated this test again later when trying to determine our bacteria, but we still obtained the same results.
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