In order to test if our bacteria would react with sucrose, glucose or lactose, we used the aseptic technique and transferred a small inoculating loopful of bacteria into a solution of each of these. We started out be sterilizing our inoculating loop, then we removed a small bead of culture from our original sample S. We flamed the opening of the tube and then put it away. We then transferred that bead of bacteria to first the sucrose solution. We stirred around the loop for a little bit to ensure that the bacteria was introduced into this new solution. Then flaming the mouth of the tube and the inoculating loop we incubated the solution at 37 degrees centigrade.
We repeated the procedure above for the glucose solution, using the aseptic technique, and then putting the tube in the incubator.
Our last solution was Lactose and we repeated this same process using the aseptic technique and incubating it at 37 degrees.
Next class we would review the results we obtained from these tests.
We discovered from the results that our bacteria utilized glucose, because it changed from a pink color to yellow.
It partially utilized sucrose, indicated by the orange-ish color, but not fully yellow yet.
We could determine that our bacteria did not utilize lactose, because it did not change at all from its original pinkish color.
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