Gelatin Hydrolysis Test

We used the gelatin hydrolysis test to determine whether our bacteria are able to digest gelatin.

First we obtained a nutrient gelatin deep tube and used the aseptic technique to inoculate the nutrient gelatin deep tube by stabbing the tube with an inoculating needle containing our bacteria from our agar slant culture.





















We the incubated the inoculate tube at 35 degrees Celsius for two days.

When we came back two days later, we took our nutrient gelatin deep tube from the incubator and placed in the refrigerator for 15 minutes. After 15 minutes, we took out our nutrient gelatin deep tube from the refrigerator and determined that it was negative. This means that unfortunately our bacteria are not able to digest gelatin.