All semester we have been doing procedures trying to figure our what our unknown bacteria was, and now we have come to a conclusion based on the accumulation of our results.
Here are our results compiled:
MORPHOLOGICAL CHARACTERISTICS
Cell Shape: Rods
Spore forming: yes
Gram Stain: positive (to variable)
Motility: motile
Capsules: no capsules
CULTURAL CHARACTERISTICS
Oxygen Requirements: facultative anaerobe
Optimum Temp.: 37 degrees centigrade
PHYSIOLOGICAL CHARACTERISTICS
Glucose: positive
Lactose: negative
Sucrose: negative
Mannitol: negative
Gelatin Liquefaction: negative
Starch: negative - We performed this procedure twice with the same results both times
Casein: negative
Fat: negative
Indole: negative
Methyl Red: positive
Voges-Proskauer (acetylmethylcarbinol): positive
Citrate Utilization: negative - We performed this procedure twice with the same results both times.
Nitrate Reduction: negative
Urease: negative
Catalase: positive
Oxidase: positive
DNase: negative
We had a hard time determining what our bacteria was, we were sure that it was gram positive, rods that had spores, but then we ran into some trouble with results from our starch and nitrate tests. We concluded from this that our bacteria must have at some point become contaminated by some other bacteria and this is why we were obtaining results that were not what was expected for our bacteria. From the information we had though.......we determined.......that our bacteria......is........
B. Cereus!!!!
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